Chloroquine diphosphate in transfection

Discussion in 'Plaquenil Generic' started by Fedorov, 09-Mar-2020.

  1. NikR XenForo Moderator

    Chloroquine diphosphate in transfection


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    Plate cells the night before to give 60-80% confluence at the day of transfection. 2. One hour prior to the transfection, change to medium containing 25µM chloroquine from x1000 stock in PBS, stored at -20°C. Volume should be 10 ml per dish. Chloroquine can be omitted, but increases efficiency about x2 3. In previous experiments that I did with and without chloroquine, I found the same result as you did that chloroquine-treated 293T cells prior to transfection was far superior than without chloroquine. Chloroquine Phosphate is the phosphate salt of chloroquine, a quinoline compound with antimalarial and anti-inflammatory properties. Chloroquine is the most widely used drug against malaria, except for those cases caused by chloroquine resistant Plasmodium falciparum.

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    Chloroquine diphosphate in transfection

    How do you do transfection for lentivirus production?, Questions with answers in CHLOROQUINE Science topic

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  5. Transfection Experiment Transfection Efficacy Reagent Volume Tissue Culture Grade Chloroquine Diphosphate. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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    Chloroquine diphosphate CQ was ingeniously used to take place of phosphate salt in traditional calcium phosphate coprecipitation method for pDNA transfection. With multiple roles of CQ in the novel Ca-CQ-pDNA complex including pDNA compaction and assistance in lysosome escape, the transfection efficiency of the pDNA was significantly increased relative to the traditional method. A severe eye problem has happened with chloroquine. This may lead to lasting eyesight problems. The risk may be higher if you have some types of eye or kidney problems. The risk may also be higher with some doses of chloroquine, if you use chloroquine for longer than 5 years, or if you take certain other drugs like tamoxifen. May 07, 2001 The chloroquine enhanced transfection procedure provides a reliable and reproducible assay of released protein and its effect is presumably mediated through its pH elevating effect on the lysosomal compartment, thereby, protecting DNA molecules from nuclease degradation.

     
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    Description: An exception occurred while processing your request. Medicines for the Prevention of Malaria While Traveling. Recommendations on Screening for Chloroquine and. Evaluation of Hydroxychloroquine Retinopathy Using Ultra.
     
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